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Title: Investigation of the interleukin-1β converting enzyme : solid phase peptide synthesis studies
Author: Urquhart, Kirstie
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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An investigation of the substrate requirements of the interleukin-1β (IL-1β) converting enzyme (ICE) has been carried out. Solid phase peptide synthesis has been used to synthesise sequences from precursor IL-1β, a cowpox inhibitor crmA, and the pro region of ICE. The peptides have been assayed to ascertain their inhibitory properties. In the course of this work an attempt has been made to establish a simple way of introducing β-turns into peptides. This has been carried out by the formation of a disulphide bridged species in which cystine formation was facilitated by the use of the turn-inducing 'ProD.Val' moiety, and by the introduction of an unnatural amino acid, cis-3-aminocyclopentane-1-carboxylic acid (ACPC). The β-turns developed in the ICE work have been introduced into a peptide containing a discontinuous epitope of gp120, and have been found, in this instance, to be interchangeable with one another, and with the γ-turn Cys-Val-Cys.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available