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Title: Prospects for monitoring spermatogenesis
Author: Turner, Katie Jane
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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The aims of the studies in this thesis were, firstly, to investigate the feasibility of detecting proteins derived from the seminiferous epithelium in testicular interstitial fluid, spermatic vein blood, testicular vein blood and also in peripheral blood. Secondly, to identify proteins might be potential markers of adverse effects on spermatogenesis. An antiserum was raised against seminiferous tubule secreted proteins and was used in conjunction with Western blot analysis to detect testicular proteins in biological fluids and also to screen a testis cDNA library to identify novel secretory products. Western blot analysis was only able to detect proteins secreted by seminiferous tubules in testicular interstitial fluid, but not in spermatic, testicular or peripheral venous plasma from adult rats. The antiserum specifically detected three proteins in testicular IF from control animals. The disruption of spermatogenesis by short-term testicular heat treatment 24 hours earlier (43°C for 30min) increased the abundance of these proteins and induced the appearance of several other less abundant proteins, all with molecular masses below 25 kDa. Two of the proteins present in IF were identified as germ cell secretory products, phosphatidylethanolamine binding protein and an androgen regulated protein (ARP-2). These results suggest that germ cell, as well as Sertoli cell, secretory products can gain access to the interstitium under both normal physiological conditions and more easily after testicular damage. The antiserum was unable to detect these proteins in peripheral blood even after heat treatment; this may be due to the insensitivity of the techniques used. However, this approach has identified two candidate proteins for the development of specific immunoassays, which will then enable more definitive assessment of whether proteins secreted by the seminiferous epithelium can be measured in blood.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available