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Title: Cytogenetic and molecular analysis of follicular non-Hodgkin's lymphoma
Author: Turner, G. E.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1992
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The incidence of the reciprocal translocation t(14;18))(q32;q21) in follicular lymphoma varies from 50% to 90% in American series. In the largest series from Europe, the translocation was present in only 21/51 (41&37) of cases. The wide variety in incidence may in part be due to the method of detection used - usually cytogenetics alone or a combination of Southern blotting and polymerase chain amplification (PCR) of DNA extracted from malignant tissue. The shortcomings of each technique suggest that a combination of all three may best determine the true incidence of the translocation. The study describes and discusses the cytogenetic analysis of lymph nodes from seventy two patients with follicular lymphoma. Forty nine patients also had DNA extracted from diagnostic specimens for molecular analysis using both Southern blotting and the polymerase chain reaction (PCR) to detect t(14;18). The translocation was found in 76% using a combination of all three methods. The most common other cytogenetic abnormalities included + 7, + der(18), + 18, + 21, + X and &43 8. Cytogenetics proved the most comprehensive method of detecting t(14;18) and Southern blotting was superior to PCR. The numerous additional karyotypic abnormalities present contributed little to the management of patients with follicular lymphoma. However, molecular methods detected t(14;18) in samples where karyotyping was unsuccessful and the stability of t(14;18) as a marker of disease was confirmed by the finding of molecular evidence of the translocation after analysis of sequential samples from several patients. The increased sensitivity of PCR allowed detection of t(14;18) bearing cells in samples of marrow and peripheral blood stem cell harvests from patients who were thought by conventional criteria to be in clinical remission. As treatment of follicular lymphoma becomes more aggressive molecular detection of t(14;18) will become more important in monitoring of treatment and detection of early relapse.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available