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Title: A new protecting group strategy for oligonucleotide synthesis
Author: Turner, Gillian
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1990
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This thesis describes the development of the various methods available for the synthesis of deoxyoligoribonucleotides. The use of the 2,2-bis (4-nitro-phenyl)ethyl group was investigated, mainly as a 5'-hydroxyl protecting group on 2'-deoxyribonucleotides, but also as a base protecting group for the O4-function of thymidinc and the Nc-exocyclic amine of adenosine. The deprotection conditions required for the removal of this group and its subsequent use in the synthesis of 2'-deoxyribonucleotides utilising phosphoramidite methodology arc described. An investigation, by 81P n.m.r., of the coupling and oxidation steps of the synthesis of DNA is included as well as a brief study of the potential use of zinc iodide as an accelerating agent in the coupling of nuclcotidcs by the phosphotriester method of synthesis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available