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Title: Aspects of the developmental biology of ovarian follicles
Author: Torrance, Colin
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1991
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Ovarian follicles are recruited from a pool of nongrowing primordial follicles and progress slowly through early phases of growth until the formation of an antrum. Little is known at present about the factors controlling follicle recruitment, preantral growth, or thecal and antral formation. This thesis reviews some aspects of early folliculogenesis and describes the development of a culture system for in vitro investigation of preantral growth. A enzymatic-mechanical isolation procedure was developed which permitted large numbers of isolated small ovarian follicle to be obtained for CBA/C57B16 hybrid mice, aged 8-10 days. The ovarian follicle is a spherical structure containing initially two main cell types - oocyte, granulosa cells with the thecal cell differentiating once the follicle has entered its growth phase. Interactions between these cell types may have an important role in controlling folliculogenesis and cell shape, cell-cell interactions and cell-extracellular matrix interactions may all influence follicular growth. To support and maintain cell shape and the spatial relationships between the cells types in the ovarian follicle a culture system involving embedding the follicles within a three-dimensional collagen gel matrix was developed. A quantitative study of isolated murine follicle growth in collagen gel culture over two weeks was carried out. The system facilitated follicle growth from small to multilaminar stages but antral formation and theca differentiation was not initiated. Cultured follicles were transferred under the kidney capsule of ovariectomized host. In vivo the follicle were able to progress to the Graafian stage. The effects of hormones and growth factors (oestrogen, follicle stimulating hormone and epidermal growth factor) on in vitro follicle development were studied using autoradiography and computer assisted image analysis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available