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Title: Control and manipulation of angiogenesis in the primate ovarian follicle
Author: Taylor, Paul
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
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Recently, techniques to quantify angiogenesis, identify putative molecular regulators, and inhibit them in vivo have become available. The work in this thesis applies these advances to the following questions: 1) What is the effect of the inhibition of the gonadotrophins, using a GnRH antagonist, on follicular angiogenesis? The hypothesis being tested was that follicular angiogenesis would be dependant on FSH/LH and be severely inhibited by GnRH antagonist treatment. The results suggest that while VEGF expression in the preovulatory follicle is under gonadotrophic control, it is not dependant on normal gonadotrophin secretion in tertiary follicles, indicating that there are other paracrine factors regulating VEGF expression in the developing ovarian follicle. The second chapter extends the findings by determining granulosa cell response to FSH stimulation with respect to induction of the VEGF and aromatase genes. 2) What is the effect of inhibition of vascular endothelial growth factor, using the antagonist, VEGF trap R1R2, on follicular angiogenesis, follicular development, ovulation and the establishment of the corpus luteum? In vivo inhibition of VEGF caused dramatic reductions in angiogenesis and in VEGF receptor expression but did not reliably prevent dominant follicle growth or ovulation once dominant follicle selection had occurred. 3) Is a novel factor, endocrine gland vascular endothelial growth factor (EG-VEGF) expressed in our animal model? Findings demonstrated that EG-VEGF is expressed in the granulosa lutein cells in the human corpus luteum while the marmoset ovary does not appear to express EG-VEGF. This thesis has improved our understanding of the gonadotrophic control of follicular angiogenesis and the role of VEGF plays in the latter stages of folliculogenesis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available