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Title: Molecular analysis and application of tissue microarray technology to the histopathological and immunohistochemical analysis of cervical adenocarcinoma
Author: Tawfik El-Mansi, M. M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2006
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A tissue microarray method yielded staining of good quality and is feasible for morphological and immunohistochemical studies in cervical adenocarcinoma. Analysis of two TMA cores achieved 100% representation for morphological studies and greater then 97% representation for immunohistochemical studies. Tissue array sections were immunostained with 8 antibodies, carcinoembryonic antigen (CEA). Cytokeratin7 (CK7), Cytokeratin20 (CK20), oestrogen receptor (ER), progesterone receptor (PgR), phosphatase and tensin homolog deleted on chromosome ten (PTEN), MIB-1 proliferation marker, and p53 suppressor gene utilizing the power vision technique for ER only and Envision technique for all other antibodies. Our findings support that all of these 8 antibodies are of potential biomarkers of a panel of antibodies for diagnosis of cervical adenocarcinomas. HPV DNA was extracted from paraffin-embedded, formalin-fixed tissues of 161 specimens of 139 patients with excluding 22 patients with the second samples and 16 normal cervical tissues. HPV DNA was detected by PCR test using type specific primers from the E6 gene and E7 gene of HPV type 16 and HPV type 18. HPV DNA was identified in 87 cases (62.6%) in which, HPV16 was positive for 65 (47%) patients and HPV18 was positive for 41 (29%) patients. Genotyping by RFLP and PCR revealed that, HPV type 16 was the most frequent type of infection comprising 46 cases (33%), followed by HPV type 18 in 22 cases (16%), and both HPV type 16 and HPV type 18 in 19 cases (14%). HPV typing in all cases of 16 normal cervical biopsies revealed negative with both HPV type 16 and HPV type 18. Our findings support that HPV 16, along with HPV 18, may play a possible role in the pathogenesis of adenocarcinoma of the uterine cervix.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available