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Title: Role of human papillomavirus and the p53 gene in cutaneous carcinogenesis in renal allograft recipients
Author: Stark, Lesley Ann
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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In this study, the prevalence of human papillomavirus DNA was examined in a range of premalignant and malignant cutaneous tumours from 52 immunosuppressed patients and 83 immunocompetent patients using Southern hybridisation analysis as a low stringency screening method and type specific polymerase chain reaction (PCR) assays for 8 HPV types. The combined results for renal allograft recipients showed that HPV was detectable in 71% of viral warts, 43% of premalignant keratoses, 42% of intraepidermal carcinomas, 51% of invasive squamous cell carcinomas and 14% of uninvolved skin specimens. The prevalence of human papillomavirus DNA was significantly greater in squamous cell carcinomas (p<0.02 χ2 test) and in intraepidermal carcinomas (p=0.05 χ2 test) than in uninvolved skin from renal allograft recipients. In immunocompetent patients, the pattern of human papillomavirus prevalence was 100% for viral warts, 15% for keratoses, 20% for intraepidermal carcinomas, 27% for squamous cell carcinomas and 8% for uninvolved skin. No single human papillomavirus type predominated in tumour specimens from either group with multiple different "high" and "low" risk HPV types being detected in both benign and malignant lesions. These data suggest that multiple human papillomavirus types may contribute to cutaneous neoplasia in renal allograft recipients and that they appear to act early in the carcinogenic process, possibly by functioning as tumour promoters via stimulation of cell proliferation. This study also employed immunocytochemical techniques to investigate the prevalence and localisation of accumulated p53 in over 200 cutaneous biopsies (including 56 squamous cell carcinomas) from renal allograft recipients and immunocompetent controls.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available