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Title: Action and toxicity of pesticides on Caenorhabditis elegans and voltage-gated ion channels
Author: Alhewairini, Saleh Sulaiman
ISNI:       0000 0004 5362 1708
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2014
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The extensive applications of insecticides in agriculture and public health require appropriate methods to monitor their ecological and toxicological effects on target and non-target organisms. C.elegans was used as a model organism in this project as it has been successfully used to assess the toxicity of environmental pollutants including those in contaminated soil. C.elegans was used here to test various concentrations of insecticides/nematicides on wild-type and mutant worms. Direct observation and counting of pharyngeal muscle contraction was carried out and showed that incubation with anthelmintic or insecticide produced a concentration dependent inhibition of pharyngeal pumping in control animals. Results obtained showed that the CCA-l T-type calcium chatmelmay be such a target for pyrethroids and DDT. Worms lacking functional CCA-l (strain JD21) were less sensitive to both DDT and deltamethrin compared with wild-type N2 worms as pharyngeal pumping was reduced by DDT in N2 and JD21 st rains with ICso values of 909.2ppm and 9942ppm respectively and by deltamethrin with ICso values of 877.5ppm and 50527ppm respectively after a 1 h treatment. JD21 won11S were also more motile compared with N2 worms. EAT-2, an acetylcholine receptor subunit, may be affected by levamisole which is an acetylcholine receptor agonist, as DA465 worms lacking EAT -2 were less sensitive to levamiso le compared with N2 especially at lower concentrations.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available