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Title: The influence of the DnaA protein on transcription of the ftsZ and DnaA genes in Escherichia coli
Author: Smith, Richard W. P.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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The investigation of the mechanisms governing the control of chromosomal DNA synthesis and cell division is fundamental to the understanding of the regulation of the cell cycle in Escherichia coli. Research over many years has shown that two proteins are central to these processes: DnaA in initiation of chromosomal replication and FtsZ in cell division. DnaA and FtsZ are not only thought to be essential to the biochemistry of these events but appear also to be involved in their timing within the cell-cycle. For this reason expression of both the DnaA and ftsZ genes are regulated by a number of different mechanisms, presumably to ensure efficient growth and division of the organism under a wide range of environmental conditions. In addition to its aforementioned role, DnaA also functions as a regulatory protein in the expression of a number of genes. Its effect is mediated by a particular recognition sequence present at its site of action, known as the DnaA-box. Such a sequence is present in the promoter region of the dnaA gene and has in the past been reported to be involved in autoregulation of this gene. In this work this putative role of the DnaA protein is reassessed and cast in doubt. The promoter region of the ftsZ gene also contains a number of DnaA boxes and some evidence exists that DnaA may be involved in ftsZ regulation. In this work evidence is presented to the contrary and it is shown that the apparent role of DnaA in ftsZ regulation is probably due regulation of the gene by a growth-rate sensitive mechanism.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available