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Title: Genes encoding sigma cross-reacting proteins of Escherichia coli
Author: Smillie, David Andrew
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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In the course of work aimed at discovering new sigma transcription initiation factors in Escherichia coli, two unknown sigma cross-reacting proteins (SCRP-23 and SCRP-27A) were identified by cross-reaction with antibodies raised against region 2.2 of sigma 70. This thesis describes the mapping, sequencing and characterisation of the corresponding genes. The gene encoding SCRP-23 was located near 652kb on the physical map of the E. coli chromosomes. Its sequence and that of part of a downstream open reading frame were determined, and found to be closely similar to the ahpC and F genes (respectively) of Salmonella typhimurium. These encode the C22 and F52a subunits of the anti-oxidant enzyme, alkylhydroperoxide reductase. The identity of the E. coli genes was further established by their ability when introduced on plasmids into an ahp deletion strain to restore cumene hydroperoxide resistance. Transcription of ahpCF was found to be driven by two promoters: ahpP1 is dependent on activation by the OxyR transcriptional regulator, whilst ahpP2 is independent of this factor. Indeed ahpP2 is located within the OxyR target site, and is repressed when OxyR is activated by oxidation. The gene encoding SCRP-27A, scrP, was located near 3416 kb on the physical map, just 250 bp downstream of arcB. arcB encodes a transmembrane sensor-regulator of respiratory functions.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available