Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.662014
Title: Enterobacterial mixed species biofilms
Author: Skillman Lucy C., L. C.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1998
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Abstract:
Green fluorescent protein (GFP) was used as a tool to examine the interactions between pairs of bacterial species. A plasmid encoding GFP from Aequorea victoria was transformed into strains of Enterobacter agglomerans (EntGFP) and E. coli ATCC 11229 (E. coliGFP). UV illumination of plates enabled species to be identified in a mixture; their spatial distribution was examined by UV microscopy; and fluorescence measurements were used to quantify adhesion and inhibition of adhesion of the strains to other cells or cell components. Cooperation between EntGFP and Klebsiella pneumoniae G1 (KlebG1) resulted in enhanced biofilm formation and alteration of dual species biofilm properties. E. coliGFP and Serratia marcescens 87b (Serr87b) stably coexisted in biofilms but did not affect the growth of each other. The other bacterial partnerships examined were competitive, with the end result that one species dominated the biofilm. Microscopic examination of EntGFP and KlebGI dual species biofilms showed that the two species were often closely juxtaposed in microcolonies, suggesting the interactions involved surface associated macromolecules. They appeared to directly interact through adhesin/receptor interactions and proteins were isolated which could form the basis of their specific interactions. In addition, EPS affected coadhesion non-specifically. Compared to single species biofilms, both species in cooperative dual species biofilms had increased resistance to disinfectants and antibiotics. Neutral dual species biofilms of E. coliGFP and Serr87b did not show increased resistance to disinfection. Therefore, the enhanced resistance was related to the specific interactions and disruption of the cooperative partnership though the integration of a third species, Serr87b, led to a decrease in resistance. The methods developed provide a convenient technique for the examination of mixed species biofilm communities where the unique interactions between species determine the true properties and resistance of the resultant biofilms.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.662014  DOI: Not available
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