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Title: The identification of plant calmodulin binding protein genes
Author: Shepherd, Jason
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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Ca2+ is a frequently utilised second messenger in higher plants. Calmodulin is a small, highly conserved, multi-site receptor for Ca2=, which interacts with a large number of downstream effector proteins. Calmodulin binding proteins (CBPs) do not have a highly conserved binding domain, and interact with calmodulin in an unusual fashion involving both a hydrophobic surface and charged domains. A number of techniques to isolate CBPs from plants were used. Techniques based on DNA homology suffer from the extreme lack of conservation between calmodulin binding domains. Other, established techniques, utilise in vitro overlays of labelled calmodulin to screen cDNA libraries, and are necessarily artificial and thus potentially artifactual. Algorithmic modelling of the calmodulin binding interaction based on up to date information was carried out using rapid, quantitative computer profiles which utilised a novel double profile technique to combine both hydrophobic and charge related patterns. This model suggests that a large number of relatively weak yet biologically significant CBPs are to be expected. The consequences of the presence of these proteins is discussed. The yeast dihybrid system was utilised as an in vivo system to screen for calmodulin binding proteins, and a number of sequences isolated and partially sequenced. These sequences demonstrate the success of this technique, and show the existence of a partially conserved calmodulin binding domain within the plant kingdom, as well as a high number of CBPs of potential biological significance.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available