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Title: Structure, expression and function of the Phantastica gene in Antirrhinum organ development
Author: Selvadurai, Harinee R. N.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1998
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The mutant phenotype of the PHANTASTICA (PHAN) gene had indicated that PHAN was required for dorsoventrality of leaves, bracts and petal lobes in Antirrhinum majus. Here it was shown that at low temperature, phan mutants carrying a likely null allele also stopped organ initiation and growth, and the shoot apical meristem arrested. These effects were reversed when plants were returned to a higher temperature. This indicated that PHAN is also required redundantly for earlier elaboration of the proximodistal axis of all organs and for meristematic function. PHAN was shown to encode a novel MYB-related transcription factor. In situ hybridisation revealed that PHAN mRNA was first expressed in leaf initials before they became morphologically distinct from the meristem. Comparisons of the expression patterns of PHAN and floral homeotic genes revealed that PHAN is expressed in floral organ initials at, or before, the time that their identifies are specified within the meristem. The early and specific expression pattern of PHAN, its requirement for elaboration of the two axes unique to lateral organs and the regulatory activity of its product suggest that it specifies the identify of lateral organ initials as distinct from the meristem and stem. The requirement for PHAN in maintenance of the shoot apical meristem appears to be non cell-autonomous and therefore to involve PHAN-dependent signalling from organ initials. To characterise the relationship between PHAN and other genes involved in meristem function, the expression pattern of an Antirrhinum homologue of the Arabidopsis SHOOTMERISTEMLESS (STM) gene, AmSTM, was determined. This showed that AmSTM was expressed in a domain complementary to that of PHAN. Evidence suggesting that PHAN is required for expression of AmSTM was obtained.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available