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Title: Characterisation of condensin subunits in Drosophila melanogaster
Author: Savvidou, Ellada
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
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In this study, I analyzed the localization and role of the CSP-D2 non-SMC condensing subunit and its effects of the stability of the condensin complex. I demonstrated that a condensin complex exists in Drosophila embryos, containing CAP-D2, the anticipated SMC2 and SMC4 proteins, and the CAP-H/Barren and CAP-G (non-SMC) subunits. CAP-D2 is a nuclear protein throughout the cell cycle, increasing in level during S phase, present on chromosome axes in mitosis, and still present on chromosomes as they start to decondense late in mitosis.  The consequences of CAP-D2 loss after dsRNA-mediated interference were analysed, and it was discovered that the protein is essential for chromosome arm and centromere resolution. The loss of CAP-D” after RNAi had additional downstream consequences on the stability of CAP-H, the localization of DNA topoisomerase II and other condensin subunits, chromosome segregation, and the dynamics of chromosome passenger and metaphase checkpoint proteins. Furthermore, even after interfering with two components important for chromosome architecture (DNA topoisomerase II and condensin), chromosomes were still able to compact, paving the way for the identification of further components or activities required for this essential process. Analysis of an SMC2 mutation showed severe chromosome abnormalities similar to those observed after CAP-D2 depletion. Chromosome organisation was compromised, sister chromatid resolution was lost and chromosomes failed to segregate properly during anaphase. Nevertheless, overall chromosome compaction was normal in the majority of mitotic cells, suggesting that the entire condensin complex is primarily required for resolution of sister chromatids and establishment of mitotic chromosome architecture rather than chromosome compaction in Drosophila.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available