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Title: Biochemical and physiological mechanisms of creatine kinase release from avian skeletal muscle during acute stress
Author: Sandercock, Dale Andrew
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1998
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Marked changes in the plasma activities of the intracellular enzyme creatine kinase (CK) have been observed in broiler chickens subjected to a range of stressful stimuli. Using a novel anion exchange chromatography technique to separate the various CK isoenzymes, it was demonstrated that the skeletal muscle form of CK (MM-CK) was predominant in plasma (96.8%) and that its activity increased 65.4% (p<0.05) in response to an episode of acute heat stress (2 h, 30oC/80% relative humidity). An investigation of mechanisms that might mediate the release of CK from this tissue was undertaken using a novel validated isolated in vitro chicken skeletal muscle preparation (m. tensor patigialis) and incubation system. CK loss from the muscle preparation under optimised control incubation conditions (150 min at 41.5oC, pH 74) was negligible, constituting less than 0.003% of total muscle CK content. Incubation at 45oC had no effect on the rate of CK loss above that observed under control conditions. Incubation under conditions of reduced O2 availability (anoxia) was without effect on CK loss from the preparation. Limiting ATP availability by impairing muscle mitochondrial oxidative metabolism induced a 4-fold increase (p<0.05) in the rate of loss of CK. Promoting external ionic calcium (Ca2+) entry into the muscle cells using 4 Br-A23187 calcium ionophore (25μM) caused a 60-fold increase (p<0.001) in the release rate of CK. Incubation with the ionophore induced a significant increase in Ca2+ accumulation (79.7%; p<0.05) as measured by the uptake of radio-labelled 45 calcium and was associated with a 8.6 fold greater total loss of CK.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available