Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.661544
Title: Epidemiology and pathogenesis of ovine pulmonary adenocarcinoma
Author: Salvatori, D.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
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Abstract:
OPA is caused by a retrovirus known as jaagsiekte sheep retrovirus 9JSRV). A unique feature of OPA is the absence of a specific humoral immune response to JRSV, despite the highly productive infection in the lungs and the disseminated lymphoid infection. JRSV cannot be cultivated on cell culture. The aim of this work is to develop tools for the diagnosis and control of the disease. The first part of the thesis has been dedicated to the production of recombinant proteins to use for immunization trials, immunological assays and immunohistochemistry techniques. Knowing the peculiar relationship between the virus and the immune system the starting point is to prove the hypothesis that immunization with JSRV can overcome tolerance and stimulate protective immune responses. Another key point in the aim of finding a vaccine for the control of OPA was to develop and animal model for the evaluation of potential vaccine preparations. The neonatal lambs’ model has been very useful to unravelling the role of JSRV and some of its pathogenesis, but because of the age of the lambs, it, clearly, is not suitable for assessment of potential vaccines. In the present study different age groups of lambs (1 month, 3 months, 6 months) have been inoculated intratracheally with the same dos of infectious lung fluid. The disease was recognised in all groups by clinical observations, gross pathology and histopathology. During the experiment viraemia was successfully detected using a novel PCR technique. Inducing OPA in older lambs and developing a method for detecting viraemia due to JSRV infection mean to move forward a system for the diagnosis and control of the disease.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.661544  DOI: Not available
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