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Title: The intracellular bacteria of the proliferative enteropathies : a comparison of in vitro and in vivo infection
Author: Sabri, Jasni
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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The intracellular bacteria from naturally occurring porcine proliferation enteropathy were cultured in an in vitro cell culture system, the morphology of the bacteria, the pathogenicity of the organism in vivo, in hamsters, and the morphological events of infection in both cultured cells and hamsters were investigated. The bacteria purified from intestinal lesions of the disease were cultured in rat enterocyte, 1EC-18 cell line. Infection was enhanced by centrifugation of the bacteria onto the cell monolayer incubated at reduced oxygen tension in a microaerobic atmosphere. However, centrifugation is not necessary for infection to occur. Bacterial infection can be passaged and maintained several times. Morphological observations of the bacteria grown in cell culture pelleted by centrifugation revealed that the bacteria measures from 0.1 to 0.3um in width and 0.7 to 2.0um in length. The bacteria are pleomorphic with a wavy trilaminar outer membrane and often indistinct cytoplasmic membrane generally clearly separated by a periplasmic space. The pleomorphic bacteria differ in internal structure and electron density of the cytoplasm; some were electron-dense and some were electron-lucent. Bacterial division which was consistently seen in the electron-lucent form occurs by transverse septation. IEC-18 cells were used as an in vitro model to study the cellular events of intestinal cell infection. Cells were either artificially infected by centrifugation or spontaneously. A method of bacterial attachment and entry into the host cell were observed in both methods of infection. In a centrifuged infection, the bacteria were seen attached by electron-dense cap projection of the cell membrane. In contrast, bacteria were seen closely opposed to the cell membrane in non-centrifuged infection. However, in both cases, attachment of the bacteria is followed by entry into and escape from endocytic vacuoles free into the cytoplasm, division and eventual extracellular release of the bacteria.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available