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Title: Genetic base broadening in tomato
Author: Saavedra Del Real, G.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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Tomato (Lycopersicon esculentum L.) is one of many autogamous crop species in which the exploited germplasm has been severely reduced as a result of the process of domestication, and particularly because the initial germplasm used to generate much of the material exploited in current varieties, represented a very small fraction of the initial variability available. The concept of genetic base broadening has been suggested as a means of mitigating this lack of diversity in modern crops, with the aim to utilise the rich genetic resources available in wild relatives, vintage varieties, and landraces. Genetic base broadening programmes involve the systematic utilisation of an arrangement of genetic variability in such a way as to generate a mass of newly adapted gene stocks available as parents in breeding programmes. This research examines options available within a genetic base broadening programme, limited by space and time. Different populations were created by hybridisation in order to examine options and feasibility within a base broadening programme. These included a study of the genetic diversity of the genus Lycopersicon, using 43 accessions of different species to examine the level of genetic variability in tomato, and the richness of diversity available in wild relatives and vintage/landrace tomato cultivars. Hybridisation was conducted as part of genetic base broadening programme to create inter-specific and intra-specific crosses between selected tomato cultivars and wild relatives. As part of possible strategies, double crosses between intra-specific populations were tested and analysed. The created populations were selfed and examined using morphological and molecular markers for polymorphism, genetic distances and heterozygosity indices from genetic population analysis computational program packages Popgene and NTSYS. Results are presented for these populations over a number of generations and reviewed against possible strategies for conservation and utilisation of this sample of populations for future breeding programmes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available