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Title: Synthesis of peptides by the solid phase method
Author: Roach, Peter L.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1990
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An investigation into the use of a novel NPROB*LEM protecting group in peptide synthesis is described. The protecting group, 2,2-bis(4'-nitrophenyl)ethoxycarbonyl (Bnpeoc), has been introduced into the full range of amino acids, including those requiring appropriate side chain protection. The Bnpeoc amino acid derivatives have been successfully applied to the solid phase synthesis of a number of peptides from ubiquitin. In addition, the peptides were assembled using a wide range of coupling reagents and conditions. The chemical syntheses of phage λ Cro protein and peptides from its DNA binding region have also been investigated, using both the novel N^PROB*LEM-Bnpeoc protecting group and the established N^PROB*LEM-fluorenylmethoxcarbonyl (Fmoc) protecting group. N.m.r. experiments have indicated that residues corresponding to the DNA recognition α-helix of the Cro protein may adopt a similar conformation in a peptide from that region.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available