Use this URL to cite or link to this record in EThOS:
Title: Effects of dietary biotin on the physiology, anatomy and mechanics of pony hoof horn
Author: Reilly, John D.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2001
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
A feeding experiment involving match-paired Treatment and Control groups of four ponies each was designed and conducted, under Home Office Project Licence number 60/01439, to test the null hypothesis that: "Dietary supplementation with biotin at a dose level of 0.12mg/Kg BW daily has no effect on the physiology, anatomy of pony hoof horn". Biotin supplementation caused a significant higher growth and growth rate of horn at the midline dead centre of the wall for Treatment compared to Control ponies (p<0.05 by students t-test). The pair of two older animals in the trial had significantly lower hoof growth when compared with the rest (p<0.05 by ANOVA). No significant difference was found between feet for these parameters and so the left fore foot of each animal was used to provide material for anatomical and mechanical studies. Quantitative methods for measuring anatomical features which may have a functional significance such as tubule density, absolute areas and area fractions of hoof wall components, were devised. These methods were then used to give normal values for these parameters in the Control group and compared with those for the Treatment group. Hoof horn stratum medium (SM) was found to consist of four distinct zones as defined by tubule density. This was new anatomical information and it was proposed that the hoof wall may act as a quadri-laminar ply. There was an effect of biotin supplementation on tubular density with treatment animals having a significantly higher tubule density in Zone 4 of the SM (P<0.05 by Mann-Whittney U Test). There was an effect of biotin supplementation on tubule marrow sizes. Treatment animals had significantly smaller mean tubule marrow sizes compared with Control animals (p<0.01 by ANOVA). This distinction was found to be due to a significant difference between Treatment and Control animals in the two younger pony pairs and the differences were found in Zone 1 of the SM only.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available