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Title: Further analysis of Brachyury (T) : a gene required during gastrulation
Author: Rashbass, Penny
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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This thesis is concerned with further analysis of the classical mouse mutant Brachyury (T). Developmental genetics has shown that this gene plays a key role in mesoderm formation during murine gastrulation. Although the Brachyury gene is only expressed in the primitive streak, nascent mesoderm and notochord of the gastrulating mouse embryo, homozygous null T/T mutants have a defective allantois, kinked neural tube and disorganized somites in addition to a bulky primitive streak and disrupted notochord. To study the cell autonomy of the T mutation, we have isolated and genetically characterised embryonic stem (ES) cell lines derived from heterozygous T/+ matings and studied their behaviour in chimaeras. T/+ ↔ +/+ form normal chimaeras whereas T/T ↔ +/+ chimaeras mimic the T/T mutant phenotype. In order to assess how Brachyury may interact with other genes which may also be required for mesoderm formation and both rostrocaudal and dorsoventral patterning, I decided to compare the expression patterns of several genes in homozygous and wildtype embryos using a non-radioactive wholemount in situ hybridisation technique. The expression pattern of three Pax genes (Pax-1, Pax-3 and Pax-6) which exhibit dorsoventral patterning in the neural tube and/or somites are altered in T/T embryos, whilst the pattern of Hox-7 which is expressed in the dorsal part of the neural tube remains unaltered. The T gene is also necessary to maintain the normal expression level of Wnt-5a and Evx-1 in 8.5 and 9.5 day embryos respectively. In contrast Wnt-3a and BMP-4 expression appear to be unaffected by the absence of the T gene product.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available