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Title: Aspects of cellular properties in the lens of the chick
Author: Randall, Fiona
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1981
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Circadian rhythms in DNA synthesis have been shown to be present in the epithelium of whole chick lenses. These rhythms are a reflection of the mitosing epithelial cells and are strain specific. Three chick strains were studied: By-l, By-2 and N. The results suggest that the cell cycle in L.B. cells is under genetic control. Synchronous cultures of L.E. have been induced in vitro by two different methods: mitotic selection and a cell cycle arrest method. Protein synthesis studies of these synchronised cells showed that no qualitative differences were apparent at different times during the cell cycle. Several instances of quantitative changes were found including an increase in 6-crystallin synthesis during S-phase and an increase in actin synthesis during the transition into S-phase. An increase in newly synthesised actin associated with the membrane was also found at this time. Few other changes in the L.E. cell membrane polypeptides during the cell cycle could be resolved by SLR-polyacrylamide gel electrophoresis. In apparent contrast, differences were found in the lectin binding capacity of L.B. cells during the cell cycle. Binding of all lectins under study was found to be greatest during mitosis. Strain differences were apparent: strains By-1 and By-2 showed greater lectin binding than strain N both during mitosis and interphase. Insulin, foetal calf serum and a retinal extract were found to induce changes in growth rate, cell morphology and crystallin synthesis in L.B. cultures of the 3 strains of chick under study. In all cases strain differences were apparent. The role of external signals to the lens is discussed in the context of lens epithelial cell differentiation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available