Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.660830
Title: Quantitative dynamics of foot-and-mouth disease virus infection in pigs
Author: Quan, M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
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Abstract:
The main objectives of this project were to provide an accurate quantitative description, and develop a mathematical model, of FMDV dynamics in infected pigs. Time-course studies were set up and the viral load in serum, nasal swabs and tissue samples were determined. Time after infection, mode of infection, inoculation dose and housing conditions were examined as possible determinants of viral load. Similar results were obtained from pigs infected with FMDV by the intravenous, intradermal or oro-nasal route. Initial models of the early viral dynamics of FMDV disagreed with the experimental data. Experimental data showed larger effects of dose on the temporal distribution of viraemia than was predicted by the models. This disagreement could be resolved by: i) limiting the rate of infection of epithelial cells at low FMDV concentrations; ii) converting the virus removal system (such as the mononuclear phagocyte system) into one of limited capacity; or iii) the addition of a virus removal system of limited capacity, such as non-specific binding of FMDV into the models. A hypothesis in which the rate of infection of epithelial cells was limited at low FMDV concentration could be supported by the literature. For FMDV to successfully infect and replicate within a cell, host protein synthesis has to be inhibited and anti-viral defences inactivated. The probability of a successful infection of a cell increases as the number of infecting viruses increases, suggesting that the rate of infection of cells at low FMDV concentrations is indeed limited. The experimental work has highlighted the strong relationship between inoculation dose and dynamics of FMDV in vivo and the modelling exercise has highlighted important determinants of viral dynamics, as well as areas where further research should be directed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.660830  DOI: Not available
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