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Title: A transgenic approach to the study of the function of the adenine nucleotide translocator
Author: Purcell, Patrick
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1993
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Binary vector constructs for Agrobacterium tumefaciens mediated transformation were prepared with both a heterologous ANT cDNA from maize and a homologous potato ANT cDNA. Both sense and antisense constructs were prepared with each cDNA under the control of the constitutive CAMV 35S promoter, and two tissue specific promoters from potato. The expression of the patatin promoter is tuber specific, whilst the light induced ST-LS1 promoter is expressed in the leaves in association with chloroplasts. A number of different protocols were used to attempt to regenerate transgenic potato plants. The overexpression of either heterologous or homologous ANT under the control of either the constitutive or tissue specific promoters did not affect the plants regenerated. The steady state level of ANT transcript was reduced in transgenic plants regenerated with both the heterologous and homologous ANT antisense genes under the control of the CAMV 35S promoter, but the amount of ANT protein was not affected. The No differences could be detected in transgenic plants with the tissue specific ANT constructs, apart from in one clone. Plants transformed with the homologous potato potato ANT antisense gene, under the control of the leaf specific ST-LS1 promoter, showed a reduction in both the level of ANT transcript and the amount of ANT protein in leaves. The expression of the antisense gene appeared to follow the expected leaf specific pattern as no change in ANT expression was found in root tissue.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available