Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.660447
Title: β-NGF and its low-affinity receptor (p75LNGFR) in benign prostatic hyperplasia and adenocarcinoma of the prostate
Author: Paul, Alan Burnett
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1999
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Abstract:
β-NGF was measured in human benign prostatic hyperplastic (BPH), adenocarcinomatous and normal tissues using an enzyme-linked immunosorbent assay. Concentrations were 1992pg/g wet weight in BPH tissue (SD = 684pg/g), 3100pg/g in adenocarcinomatous tissue (SD = 1503pg/g), and 2690pg/g in normal tissue. mRNA transcripts for β-NGF were demonstrated in prostate tissues by reverse transcription-polymerase chain reaction showing that the β-NGF measured in prostate tissue was endogenously produced. Western blotting allowed the demonstration that immunoreactive β-NGF in the prostate represented dimeric β-NGF and not behaviour β-NGF-like proteins which have been described elsewhere. Immunohistochemistry for β-NGF localised the hormone to the prostate epithelium in BPH, cancer and normal tissue. This epithelial localisation was confirmed by video-assisted tissue morphometric studies. Thereby it was shown that specimen β-NGF concentrations correlated well and statistically significantly with specimen prostatic glandular content. Morphometric studies also allowed the expression of β-NGF concentrations in terms of each specimen's contained, β-NGF secreting, glandular tissue. Thereby it was demonstrated that BPH glandular tissue produced greater concentrations of β-NGF (1597pg/100mg glandular tissue, SD = 788pg/100mg) than did malignant glandular tissue (1058pg/100mg glandular tissue, SD = 559pg/100mg), in spite of the higher concentrations of β-NGF found grossly in adenocarcinomatous tissue. β-NGF concentrations did not correlate with differential of adenocarcinomas or with the degree of neurodocrine differentiation therein. The thesis suggests that human prostatic β-NGF has a role similar to that described in other tissues. That is the maintenance and stimulation of adrenergic nerves. This may be relevant to the widespread use of sympatholytic drugs in the treatment of BPH.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.660447  DOI: Not available
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