Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.660438
Title: The chemical synthesis of proteins and peptide C-terminal derivatives
Author: Patterson, Jennifer Ann
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1999
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Abstract:
Methodology for the synthesis of peptide C-terminal aldehydes has been investigated. Modification of a linker system, based on a terabenzosuberyl construct, has been demonstrated to be suitable for the synthesis of peptide C-terminal semicarbazones. The route has been fully optimised to yield a series of peptide C-terminal semicarbazones and the corresponding peptide aldehydes. The chemical synthesis of deglycosylated human interferon-gamma (143 residues) has been carried out. The purification of this protein has been investigated and a short purification protocol developed which is sufficiently general to allow application to other similar protein systems. Purification and characterisation of the synthetic interferon-gamma molecule has been completed and folding of the molecule attempted.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.660438  DOI: Not available
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