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Title: Cell surface studies in normal and abnormal chick lenses
Author: Odeigah, Peter G. C.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1977
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The lens cell membranes and cell surface properties of two genotypes of chickens (HY-1 and HY-2) selected for high growth rate and associated with hyperplasia of the lens epithelium have been studied using a normal genotype as control. The results indicated that HY-1 and HY-2 cells are characterised by abnormalities in the structure and composition of their cell membranes. The membrane protein polypeptides differ markedly from each other both by charge and size. HY-1 and HY-2 membranes also have a high sialic acid content. Ultrastructural studies showed that both the intact epithelial cells and the membrane fractions of HY-1 and HY-2 cells have a marked deficiency of gap junctions. Immunological studies showed that although the membrane components of these cells share some antigenic properties with those of the normal, they are however not identical. Studies of lens epithelial cultures showed that these cells display numerous cell surface microvilli in contrast to their normal counterparts. It is suggested that this may be related to their increased lectin-binding and agglutinability, as revealed by studies with lectins, and their high metabolic rate as shown by their incorporation of radioactive precursors. Polysomes synthesising membrane components in these cells were studied by immunoprecipitation and the results revealed that these polysomes have a faster turnover than their normal counterparts. These cells bound more 45 calcium than normal cells but the activity of Na+-K+-ATPase in the lens epithelium of these cells was not significantly different from normal. It is suggested that the cell membrane modifications in these cells may be related to their abnormal cellular behaviour in cell culture and the hyperproliferation of the lens epithelium in vivo.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available