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Title: Synergistic activities of the selected antibiotics of fluoroquinolones, β-lactams, aminoglycosides, glycopeptides and streptogramins against gentaminic resistant Enterococcus faecalis and Enterococcus faecium
Author: Obwona, B.-A.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
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Abstract:
The aim of this study was to investigate synergistic activities of the combined antibiotics against the gentamicin resistant Enterococcus faecalis and Enterococcus faecium and to establish the existence of some genes involved in the resistance of enterococci against gentamicin and ciprofloxacin. The total of 81 clinical isolates were collected for the study and all were found to be resistant to gentamicin (MICs range 32->256mg/l). 50 isolates were found to be resistant to ciprofloxacin with MICs range 64->256mg/l. The synergistic activities of the antibiotics combinations were establish against E. faecalis and E. faecium Clinical isolates. The combination between Amoxicillin and gentamicin against E. faecalis resulted into synergy with MIC at 90% of 0.5mg/l and for E. faecium, MIC at 90% was 2mg/l. Vancomycin and gentamicin combination had MIC at 90% of 2 mg/l for E. faecalis and 1mg/1 for E. faecium. Teicoplanin and synercid combination showed synergistic activity of MIC at 90% of 0.5mg/l for E. faecalis while E. faecium had also 0.5mg/l. The combination between teicoplanin and ciprofloxacin had synergy with MIC at 90% of <0.25mg/l for E. faecalis and E. faecium had the same activity of MIC at 90% of <0.25mg/l. The combination between amoxicillin and synercid resulted into synergistic activity at MIC 90% of 4mg/l for E. faecalis and also 4mg/l for E. faecium. The antagonistic activity was observed between combination of vancomycin and synercid with MIC at 90% of 32mg/ for E. faecalis and MIC at 90% of 16mg/l for E. faecium. The combination between Synercid and ciprofloxacin resulted into synergistic activity at 90% MIC of 4mg/l for E. faecium. The checkerboard test of the combination between synercid and ciprofloxacin against four clinical isolates of E. faecium resulted into FIC indices of 0.4 for isolate D002 and 0.4 for isolate G051. While isolate 788/5/95 had 0.3 and NCTC12202 had also 0.3. These indices confirm the synergistic activity of the combined antibiotic of the two drugs. Of 27 isolates tested for the presence of aminoglycoside modifying enzymes using PCR technique, only 8 (2 E. faecium and 6 E. faecalis) showed the positive results of the presence of AME. However, the presence of the AME did not prevent the synergistic activities of the combined drugs against E. faecalis and E. faecium. The PFGE study showed the heterogeneous existence of these gentamicin resistance isolates from RIE.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.660156  DOI: Not available
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