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Title: OPCML and the IgLON family : expression, function and links with ovarian cancer
Author: Ntougkos, Evangelos
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2006
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Expression studies of OPCML and the other IgLON family members were undertaken in cancer cell lines, as well as normal mouse and human tissues. Expression analysis of the IgLONs in the two OPCML-transfected cell line systems identified a transcriptional NEGRI at the RNA level. In the mouse and human, the expression profile of the family was established in panels of multiple-tissue cDNAs, where similarities but also differences among different IgLONs were highlighted. Immunohistochemical studies of OPCML were used to profile expression developmentally and in adult tissues. A comparison between IgLON RNA levels in human normal ovaries and a panel of ovarian tumours has pointed to significantly reduced levels of OPCML, LSAMP and NEGRI in specific histological subtypes of ovarian cancer; HNT expression, on the other hand, was significantly elevated. This study has revealed the importance of the IgLON family as a whole in EOC. Various assays were undertaken in the two transfected cell line systems in order to suggest potential functions of OPCML. In SKOV-3 cells, OPCML was shown to significantly increase invasion through matrigel, and increase adhesion to vitronectin. Moreover, OPCML was found to promote cell-to-cell adhesion. The growth suppression effect of OPCLML in vitro was reproduced, and the underlying mechanism was investigated. This effect is not accounted for by a difference in proliferation; on the contrary, OPCML was shown to significantly increase apoptosis.  Expression of OPCML in the non-ovarian HeLa inducible cells did not recapitulate the phenotypic features identified in the ovarian SKOV-3 cells, indicating the importance of context specificity.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available