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Title: Escherichia coli O157:H7 infection in cattle : shedding patterns, tissue tropism and pathological immune responses
Author: Nart Peñalver, P.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2009
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Recent research has established that the terminal rectum is the predominant colonization site for Enterohaemorrhagic E. coli O157:H7 in cattle. Thus the main goals of the research were to understand the carriage and persistence of E. coli O157:H7 in the bovine host, through examination of faecal shedding patterns and gastrointestinal tract tissues in experimentally infected calves. A marked tropism for the terminal rectum has been confirmed while other minor sites were identified. E. coli O157:H7 induced histopathological alterations of the rectal mucosa. These pathological changes induced a local infiltration of neutrophils and rectal mucosal IgA responses against E. coli O157:H7 antigens. The work presents evidence of local IgA immune responses directed against membrane protein components, secreted Type III proteins, O157 lipopolysaccharide and H7 flagellin. This study has sought to elucidate the mucosal immune responses against E. coli O157:H7 outer membrane porine C as a specific antigen; in particular against the sections of the protein situated on the external surface of the bacterial membrane. While there were consistent immune responses against the whole membrane protein, purified peptides of the external areas of the porine failed to elicit detectable mucosal immune responses. An understanding of the immune responses will support the development of diagnostic tests and control methods, such as vaccines to limit carriage in the bovine host.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available