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Title: Investigation into the intracellular mechanisms involved in prostaglandin synthesis by, and release from, the guinea-pig uterus
Author: Naderali, Ebrahim Khalil
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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Caffeine (10 mM) stimulated the outputs of prostaglandin (PG) F, PGE2 and PGI2 (measured as 6-keto-PGF) from the day 7 and day 15 guinea-pig uterus superfused in vitro. Caffeine-induced PG production was unaffected by the removal of extracellular calcium, ryanodine (RY) and ruthenium red (inhibitors of calcium release from ryanodine receptor (RYR) channel, and calmodulin inhibitors (W-7 and trifluoperazine (TFP)). In fact, W-7 greatly potentiated the caffeine effect on PGF output. TMB-8, an intracellular calcium antagonist, inhibited the increase in PGF output produced by caffeine without preventing the increases in outputs of PGE2 and 6-keto-PGF. Caffeine (1 mM but not 0.1 mM) and theophylline (Theo.; 10 mM) also stimulated PG outputs from the day 7 guinea-pig uterus superfused in vitro. Caffeine and RY both stimulated prostaglandin production by the perfused mesenteric vascular bed of the rat. Caffeine (10 mM) stimulated the output of PGF after 8 h, and the output of 6-keto-PGF after 2, 8 and 24 h from the day 7 guinea-pig endometrium in culture. Caffeine (10 mM) inhibited PGF output after 2 h, but stimulated PGF output after 24 h from the day 15 guinea-pig endometrium in culture. The amounts of PG released from cultured epithelial cells were between 100- to 200-fold and 300- to 1000-fold higher than those from cultured stromal cells from the day 7 and day 15 endometrium, respectively. Outputs of all three PGs from epithelial cells, but not from stromal cells, were 4.4- to 5.5-fold higher from cells obtained on day 15 than on day 7. PGF was the major PG released from the epithelial cells obtained from day 15 guinea-pig uterus. Caffeine had both a stimulatory and an inhibitory effect on PG output from endometrial cells cultured from the day 7 and day 15 guinea-pig uterus.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available