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Title: Studies on suramin resistance in Kenyan stocks of Trypanosoma evansi (Steel, 1885 Balbiani, 1888)
Author: Mutugi, Marion Wanjiku
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1993
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The work described in this thesis examined 44 stocks of T.evansi, 41 of which were isolated from camels in various areas of Kenya. These trypanosomes had a mean length of 25.5 ± 2.8 μm a mean pre-patent period of 4.7 ± 3.4 days and fulfilled the classical criteria of this species in regard to these two parameters. Initially, the sensitivity of these trypanosome stocks was determined to four trypanocidal drugs active against T.evansi. The 44 stocks exhibited resistance to Berenil (57%), Samorin (7%), suramin (22%) and Trypacide (18%). It was noted that although Berenil is not recommended for use in camels due to its toxicity, more than half of the stocks were resistant to this drug. The malic enzyme patterns of these stocks were determined to investigate possible correlation with resistance to the trypanocides used. Most of the stocks (66%) possessed malic isoenzyme pattern II (66%), although patterns X (23%), IV (7%) and VII (5&37) were also observed. Malic enzyme patterns IV and X had not been identified previously in this trypanosome species. No linkage was found between any of these patterns and resistance to the four drugs. Further work concentrated on three trypanosome stocks which were highly resistant, of intermediate resistance or sensitive to the action of suramin. Investigations on the effect of trypanosome inoculum and timing of suramin administration in obtaining cures in T.evansi infected mice were carried out. The time of administration after infection was shown to be an important factor that influenced the outcome of suramin treatment. Mice which were treated immediately after infection had higher cure rates than those treated at the onset of parasitaemia. This was observed particularly in a trypanosome stock normally resistant to suramin at a dose rate of 160 mg/kg; if treatment was administered immediately after infection, most of the infected mice were cured. Except in the highly resistant stock, the role of trypanosome inoculum was not as important as timing of treatment in determining cures.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available