Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659070
Title: Anatomical and functional study of parvalbumin-positive interneurons in the hippocampal formation
Author: Foggetti, Angelica
ISNI:       0000 0004 5358 381X
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2014
Availability of Full Text:
Access through EThOS:
Full text unavailable from EThOS. Please try the link below.
Access through Institution:
Abstract:
It has long been acknowledged that inhibitory interneurons play a crucial role in regulating the input-output functions of principal cells in the hippocampus. The investigations we conducted focus on one specific population of interneurons, expressing the protein parvalbumin. The thesis describes three different studies, aimed to characterize anatomical and functional aspects of parvalbumin positive interneurons in the mouse hippocampal formation. The first study examines long-range projections of these neurons from CA1 and subiculum to distant regions of the brain, finding potential targets mainly in septal, thalamic and hypothalamic areas. The second study investigates the role of parvalbumin-positive interneurons of the dentate gyrus in spatial memory. Behavioural experiments with radial arm and Morris water maze have been carried out to understand how these GABAergic interneurons regulate information flow during reference and working memory. Finally, a third study describes basic anatomical features of parvalbuminpositive dendritic spines in the dentate gyrus. Their characteristics have been widely studied in principal neurons but little is known about spines in interneurons. Here I show a peculiar distribution of spines on apical dendrites of these cells, with a predominant localization within the inner third of the molecular layer. All studies utilized a combination of transgenic Cre-expressing mice and Creactivatable AAVs. For the first and third study AAV-based neuronal labeling was applied to visualize neurons, including their projections and their spines, respectively, through expression of fluorescent proteins. For the second study instead two genetic tools have been used in order to disrupt the neurotransmission from parvalbuminpositive interneurons and examine the effects on behavioral task performance.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.659070  DOI: Not available
Keywords: Interneurons ; Hippocampus (Brain)
Share: