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Title: Isolation and characterisation of genes involved in mammalian eye development
Author: Monaghan, A. Paula
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1990
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Vertebrate eye development requires the coordinate specification of a number of tissues and cell interactions which produce the precisely assembled organ of the adult. Careful observations of the developing eye in a variety of organisms, in addition to detailed descriptive cataloguing of its morphogenesis have provided a number of clues to the forces which direct its development. However, the advent of molecular biology has allowed analyses of the underlying forces which drive these events on a much finer level. The purpose of this project was therefore to isolate genes which are involved in eye development or maintenance, since relatively few genes have been identified which are involved in these processes. The identification of two groups of genes which are involved in programming developmental decisions has greatly aided the understanding of events which occur at the genetic level. These genes are the homeobox containing proteins and the zinc finger proteins which contain domains which are directly involved in specific DNA interaction. Originally identified in developmental and transcription regulators, the conservation of these DNA binding domains has provided an excellent tool to isolate similar genes from a variety of species. The role of three such genes has been characterised in the mammalian eye. A gene has been isolated from a human retinal cDNA library whichcontains a number of zinc-finger motifs. The expression of this gene (Rox5), is detected at low levels in all tissues examined. The level of expression varies between individual tissues with maximum expression in tumour cells. In serum deprived cells, the transcription of Rox5 and a related gene is rapidly induced approximately five fold. Based on these observations, it is proposed that this gene is a transcriptional activator, which is stimulated in response to low serum conditions in cells. It is possible that this gene is a member of the stress protein family which are rapidly induced in response to various stimuli in all cells in the body.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available