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Title: Immune responses in a mouse model of Lawsonia intracellularis infection
Author: Mitchell, Suzanne Christina
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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The aim of this thesis was to establish an infection system in the mouse and use this to examine the development of infection and lesions of proliferative enteropathy within the gastrointestinal tract. This mouse model was then used to assess the mucosal and serological responses generated by the host in response to inoculation with L. intracellularis. The infection system demonstrated that 129/Sv/Ev wild-type and isogenic interferon gamma receptor null (IFNγR-/-) mice developed characteristic lesions of proliferative enteropathy following oral challenge with a pure culture of L. intracellularis (approximately 5xl07 organisms). These were directly comparable to the lesions seen in natural disease with increased proliferation of infected enterocytes in the ileum and colon associated with the loss of goblet cells. Wild-type 129/Sv/Ev mice appeared capable of resolving or resisting infection with this obligate intracellular pathogen, with lesions of proliferative enteropathy detectable only at day 14 or 21 post inoculation, thereafter (days 28 and 35) there were none detectable. In comparison, IFNγR-/- mice were highly susceptible to disease and appeared unable to eliminate infection over the time course studied, indicating that a functional IFNγ receptor is necessary in the control of infection. This novel system is the first detailed description of proliferative enteropathy in mice and has demonstrated the usefulness of this model in the characterisation and analysis of important immunological events during infection with Lawsonia intracellularis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available