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Title: The transition from vegetative to floral development in the shoot apex
Author: Miller, Malcolm Barry
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1976
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During this work a study was made of changes in the shoot apex during floral transition. A plant (Silene coeli-rosa L.) in which the shoot apex transforms directly from leaf production to floral-part production, was used. Silene is a LD plant and will flower if exposed to 4 or more LD. It has been shown that during, induction the growth rate of the apex is the same as in vegetative plants. During early flower morphogenesis however, both the cell cycle of the apical cells lessened and the proportion of dividing cells in the apex increased. Following the start of induction the first change (apart from perhaps a slight stimulation of growth following exposure to 1 LD) recorded in the apex was a rise in the concentration of RNA. If fall induction took place this increased RNA concentration was maintained. Following sub-inductive LD treatment on ephemeral rise in RNA concentration took place in the apex. This suggests evocation in Silene is a stepwise phenomenon. The effects of sub-inductive LD treatment remained in Silene for up to 4 days. The concentration of RNA in the apex rose, in the absence of any change in growth rate, following GA application suggesting the RNA rise in induced plants may result from the arrival of hormone(s) in the apex. On transfer to cold conditions both the RNA concentration and the growth rate in the apex fell. Induction was unaffected by cold conditions and it was shown the RNA concentration and the growth rate was similar in the apices of vegetative plants at 20°C and plants producing flowers at 12°C. This suggests the maintenance of high RNA levels may be linked to the increases in growth rate which normally takes place during flower morphogenesis. Although the overall protein concentration in the apex was shown to innrease in Silene during flower morphogenesis no evidence could be found for any change over this period in the electrophoretic pattern following polyacrylamide gel electrophoresis of apical protein samples.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available