Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.657772
Title: Radiochemical purity of technetium-99m radiopharmaceuticals : measurement by high performance liquid chromatography
Author: Millar, Alistair Mackay
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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Abstract:
High performance liquid chromatography (HPLC) has been reported as a means of demonstrating the multicomponent nature of 99mTc radiopharmaceuticals. HPLC has not, however, been widely reported as a satisfactory technique for measuring the radiochemical purity (RCP) of 99mTc radiopharmaceuticals. Inadequate techniques based on thin-layer and paper chromatography are in widespread use for this purpose. The aim of the work described in this thesis was therefore to carry out a scientific investigation to establish if quantitative HPLC is a technique that can be used to measure the RCP of 00mTc radiopharmaceuticals. An on-line radiation detector was developed and evaluated. This was constructed from a length of column outlet tubing positioned inside the well or across the face of a sodium iodide crystal scintillation detector. The different configurations of tubing and crystal were used to create different sensitivities of detection. Chromatograms were recorded with either a counter-ratemeter/printer system or a computer-based multichannel analyser. At 6,000 counts per second, the highest count-rate encountered in the course of the work, the loss due to dead-time in the multichannel analyser was satisfactorily low at 0.5%. For successful measurement of RCP, none of the radiolabelled species in a radiopharmaceutical must be retained on the HPLC column and thereby escape detection. A technique for measuring recovery from the column was therefore developed. This involved injecting the sample onto the column with a loop-valve injector, collecting the eluate and measuring the count-rate from it relative to the count-rate from an accurately pipetted sample of the radiopharmaceutical.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.657772  DOI: Not available
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