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Title: Taxonomy to underpin cultivar identification in hoop-petticoat daffodils
Author: Könyves, Kálmán
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2014
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Cultivars are traditionally identified by morphological and phenological characters during the growing season. However outside of the growing season and with an enormous number of cultivars this is often unfeasible. Molecular techniques, especially microsatellite analysis, offer potentially effective and cheap methods for cultivar identification. Complex breeding programmes of daffodils over the past 150 years have resulted in more than 30,000 cultivars. Despite the economic importance of daffodils the taxonomy of the genus is unresolved. This thesis aims to evaluate the use of microsatellites in Narcissus for cultivar identification and taxonomic revision, in combination with DNA sequencing, using hoop-petticoat daffodils as a model system. Three field trips were conducted to discover the natural variation within section Bulbocodii. A total of 455 samples were analysed from across the natural range. Twenty-four Division 10 cultivars and five wild-type hooppetticoat daffodils were sourced from commerce. A new microsatellite library of eleven loci was developed. All of the hoop-petticoat daffod ils could be genotyped. The transferability of the microsatellite markers was tested on 18 additional daffodil species. Transferability ranged from 39% to 100%. A part of matK was sequenced to help identify hybrids both in the wild and amongst cultivars. Of the naturally occurring species Narcissus bulbocodium, N. hedraeanthus and N. obesus could be identified. The remaining two species N. cantabricus and N. romieuxii could be separated from the other taxa, but not from each other. Twenty-two out of the 24 cultivars could be identified. The molecular markers proved useful in the identification of mislabelled plants. The microsatellites were also tested on 54 non-Division 10 cultivars. Eight out of 11 microsatellite loci could be transferred to at least 94% of the samples. Additional microsatellite markers couid be developed to provide resolution for over 30,000 daffodil cultivars. This project demonstrated that microsatellite markers could allow an objective evaluation of the taxonomic diversity of hoop-petticoat daffodils and their cultivars.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available