Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.657334
Title: Ca²⁺ signalling in response to mechanical perturbation and hypo-osmotic shock in Neurospora crassa
Author: Marris, Peter
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2007
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Abstract:
The aims of this research were to analyze the Ca2+ and physiological responses to mechanical perturbation and hypo-osmotic shock in Neurospora crassa. Cytosolic free Ca2+ concentration ([Ca2+]c) was measured by expression of codon optimized aequorin in wild type and deletion mutants in which genes encoding different components of the Ca2+ signalling machinery had been deleted. The [Ca2+]c responses of germ tubes, vegetative hyphae and conidia were characterized. Ca2+ signatures produced in response to mechanical perturbation or hypo-osmotic shock were analysed to identify which components of the Ca2+ signalling machinery were responsible for generating these signatures. The involvement of multiple proteins in the [Ca2+]c responses to mechanical perturbation and hypo-osmotic shock of germ tubes was identified. The Ca2+ signature and germ tube swelling produced in response to mechanical perturbation were both dependent on the influx of external Ca2+ and the MID1 mechanosensory protein. The plant antifungal proteins (defensins), MsDef1, RsAFP2, MtDef2, and MtDef4, were all found to have distinct, stimulus specific effects on the [Ca2+]c responses to mechanical perturbation and hypo-osmotic shock. The mycovirus antifungal protein KP4 exhibited no inhibitory effect on the [Ca2+]c response to either stimulus. This analysis provided the basis for the development of a high throughput assay for the discovery of antifungal compounds that target Ca2+ signalling and homeostasis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.657334  DOI: Not available
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