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Title: Epidemiology and pathogenesis of fasciolosis in eastern Nepal
Author: Mahato, S. N.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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In part one, epidemiology of fasciolosis in eastern Nepal, a 19 months field survey on the epidemiology of fasciolosis is described. Four Lymnaea spp; L. auricularia race rufescens, L. auricularia sensu stricto, L. viridis and L. luteola were identified. L. auricularia race rufescens was the predominant species. The snail main habitats were spring or stream fed rice-fields, irrigation channels, ponds and road-side pools. The monsoon rains and rice cultivation practices contributed to the creation and expansion of the habitats. The snail population density was high during the dry period and declined with the onset of the monsoon. Snail egg masses and young snails were observed throughout the year. Mature Fasciola spp, infections were found in the hills from May to February and throughout the year in the Terai. In part two, experimental studies on pathogenesis of fasciolosis with special reference to its effects on productivity of ruminants is described. Monitoring included clinical, parasitological, haematological, biochemical and pathological observations. Pilot comparative studies in Scottish Blackface and Suffolk cross sheep conducted in Edinburgh indicated that F. gigantica was more pathogenic than F. hepatica. In another pilot experiment in Nepal using local Baruwal sheep, it was also found that very low infections with F. gigantica caused measurable production losses. Pathogenesis in goats was investigated using Nepalese hill goats. Infection caused production losses including weight loss. Burdens of more than 1.3 flukes/kg of initial liveweight produced clinical chronic fasciolosis. In part three, the relative merits of the current methodologies for speciation and differentiation of Fasciola spp. are reviewed. The development of species-specific (MHFh and MHFg) and cross-reactive (MHFx1 and MHFx2) DNA probes for the identification of Fasciola spp. are described. If used in conjunction these probes clearly differentiate F. hepatica and F. gigantica.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available