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Title: Germ cell modulation of Sertoli cell function
Author: Maguire, Sharon Marie
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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The aim of this work was to assess the influence of germ cells on the expression of selected Sertoli cell mRNAs. To this end, adult rats were treated with 650mg/kg methoxyacetic acid (MAA) to induce the specific depletion of >80% of pachytene and later spermatocytes from most tubules, and expression of selected Sertoli cell mRNAs was then assessed at various times after treatment when particular germ cell types were depleted selectively (see Bartlett et al. 1988; Allenby et al., 1991). Studies on the expression of cyclic protein 2(CP-2) mRNA supported the hypothesis that germ cells can influence the cyclic function of Sertoli cells. Expression of CP-2 mRNA was shown by Northern blot analysis to decrease significantly 21 days after MAA treatment. In situ hybridisation showed that CP-2 mRNA expression was decreased or absent from tubules at stages at which CP-2 mRNA is normally expressed (stages IV-VII) when elongate spermatids were depleted selectively from these tubules. This decrease was reflected in loss of CP-2 protein production. These observations lead us to hypothesise that elongate spermatids positively modulate CP-2 expression in the Sertoli cell, with this modulation occurring at the level of transcription. In conclusion, this study demonstrated that germ cells do appear to influence Sertoli cell gene expression. This can occur at the level of transcription as was demonstrated by the effect of elongate spermatids on CP-2 mRNA expression, or may occur post-transcriptionally, as would appear to be the case with ABP.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available