Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.657078
Title: Human lymphoid cell immunophenotypes : in situ immunochemistry of human lymphoid tissue, benign and malignant cutaneous lymphohistiocytic infiltrates with monoclonal antibodies
Author: McMillan, Euan Murray
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1990
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Abstract:
This thesis describes the results obtained with 36 monoclonal antibodies (McAbs) and immunocytochemistry on a) non-malignant lymphoid tissue [thymus (1) (n) = number of specimens tested, lymph node (5), tonsil (15)]. b) 104 skin biopsies of cutaneous lymphoma (40), contact dermatitis (14), parapsoriasis (17), chronic inflammatory dermatosis (9), non-lymphomatous erythroderma (3), atypical lymphocytic infiltrate (3), granulocytic sarcoma (1), lymphocytoma (3), lymphomatoid papulosis (3), lymphocytic infiltrate of Jessner (1), chronic lymphocytic leukaemia (1), myelomonocytic leukaemia (1), histiocytosis X (3), and c) normal scalp (5). In lymphoid tissue, determinants against T, and B cells (and their subsets), macrophages /D cells, and K/NK cells have a distinct topography. Immature thymic determinants OKT6, OKT9, OKT10 are identified in extrathymic locations (tonsil, and/or inflammatory infiltrates as well as lymphomas). OKT6 cross reacts with epithelial dendritic (Langerhans') cells. J5 (common acute lymphoblastic leukemia antigen) is absent in lymphoid tissue but present in some cases of mycosis fungoides. Immature myeloid markers (My10, My12) are absent in lymphoid tissue. The so called leukaemia/lymphoma markers Bel/Be2 react with follicular epithelium (Be1), dermal endothelium (Be2), tonsil (Be2), benign dermatoses (Be1 and Be2) as well as most lymphomas. A mature helper T cell phenotype is usually present in cutaneous T cell lymphoma (CTCL) and inflammatory dermatoses. However, OKT9 and OKT10 are preferentially expressed in CTCL versus parapsoriasis and benign infiltrates. Lymphomas of non-mycosis/Sezary type can be categorized into T, B and U types. They often show aberrant differentiation when examined with multiple markers. Pseudolymphomas, sarcoidosis, leukaemia and histiocytosis X show characteristic phenotypes denoting the expansion of selected lymphohistiocytic subpopulations. The histiocyte of histiocytosis X predictably expresses the OKT6 marker, but in addition reacts with the Leu3A (helper T cell) antibody. Characteristic dendritic cell markers are associated with B (R423+ ) and T (OKT6+ ) populations in lymphoid tissue and skin. K/NK cells are identified in benign and malignant infiltrates. This may have immuno-pharmacological application. The markers used amply illustrate the heterogeneous nature of benign and malignant lymphoid populations.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.657078  DOI: Not available
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