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Title: Primordial germ cell development in the chicken Gallus gallus domesticus
Author: MacLeod, David J.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2007
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Gene homologues were identified by carrying out BLAST searches of the chicken genome and EST databases. Following identification, an expression profile of each gene was obtained using RT-PCR, Northern and in situ hybridisation analyses carried out on embryos at stages of embryonic development which PGC specification; PGC migration to the gonads and PGC development in the gonads occurred. Analyses focused on the genes bruno, germ cell-less, oct4, mago nashi, nanog, nanos, piwi, pumilio and staufen and aimed to determine (1) whether gene expression was comparable to the expression of homologues in other species, (2) whether the gene was expressed in areas known to contain PGCs, (3) whether the gene was expressed specifically in PGCs and (4) whether the gene could have a potential role in PGC development. Of the genes chosen to be investigated, homologues of bruno, germ cell-less, mago nashi, piwi, pumilio and staufen were identified in the genome and in EST databases. No nanos homologues were identified by this method. Homologues of oct4 and nanog were identified by Dr B.Pain who provided sequence data for both genes. analyses showed that the expression of all the genes were comparable to expression patterns in other species. Nanog and oct4 were expressed in a pattern that indicated germ cell specific expression. This led to additional in situ hybridisation, PGC transfer and qPCR analyses at later stages of development. These data showed how expression of oct4 and nanog changed in relation to (1) the loss of PGC motility in males and females and (2) the onset of meiosis in females. Of the remaining genes, germ cell-less and piwi were detected specifically in adult testes, whereas the rest were detected in areas of the embryo known to contain PGCs. However, the gene expression was not PGC specific.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available