Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.653909
Title: Characterisation of two genes expressed during Drosophila oogenesis and embryogenesis
Author: Lin, Shengyin
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2003
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Abstract:
The interaction between somatic follicle cells and germline cells during oogenesis establishes the anterior/posterior and dorsal/ventral axes of the oocyte and future embryo. Identification of novel gene expression in groups of follicle cells at specific stages of oogenesis may therefore help us to understand pattern formation in Drosophila oogenesis. The novel gene Z14/Dmbves was identified as part of a GAL4/UAS enhancer trap screen. The Z14/Dmbves gene is similar to a gene encoding blood vessels/epicardial substance (bves) in Chicken and humans. We found that Z14/Dmbves is expressed in the nurse cell and all the follicle cells surrounding the oocyte except those in the anterior-dorsal and posterior regions, which receive the gurken (Grk) signal from the oocyte during polarity establishment. Further analysis indicated that the expression of Z14/Dmbves is repressed by the Grk signal. P-element mobilisation to generate mutants was undertaken but no mutants of Z14/Dmbves were obtained. Investigation of transgenic flies revealed that disruption of the function of Z14/Dmbves caused lethality to both embryos and larvae. Our data suggests that Z14/Dmbves might still be a lethal gene and is crucial for embryonic development. The ntf-2 gene was identified as part of a P-element mobilization screen for generating mutants of Z14/Dmbves. We propose that ntf-2 is essential for the nuclear translocation of the Dorsal protein, which is a crucial step in the establishment of dorsal-ventral patterning during Drosophila embryogenesis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.653909  DOI: Not available
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