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Title: Combinatorial polymer synthesis and inkjet printing for cellular control and manipulation
Author: Liberski, Albert Ryszard
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2009
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Inkjet printing was used for the fabrication and optimization of polymer microarrays for high-throughput screening of small molecule polymorphisms. In particular, inkjet printing was used for polymer deposition in a microarray format and for the dispensing of small molecules in solution. Crystals formed on polymer spots were screened for recording of the polymorphism. Moreover, inkjet printing was used for performing high-throughput polymerization. In the in situ nL scale polymerization the homo and copolymers were fabricated directly in microarray format. Prepared polymer microarrays were screened to identify polymers suitable for mouse embryonic stem cell adhesion and growth. To advance cell patterning in various non-microarray formats the strategy based on preferential cell binding on collagen was applied. Collagen was dispensed by inkjet printing in patterns laid-down by a bitmap converter. The second strategy for advance cell patterning was based on a simple masking process. A laser printer was used to generate a non-binding surface on glass. This simple concept delivered excellent results. Finally, polymer microarray screening was used to develop a platform for human cornea epithelial cell transfer. In this case, 252 polymers (polyurethanes and polyacrylates) were screened in a culture of cells. A transfer experiment was performed to prove the ability of cells to migrate from the cultivation surface to the target surface. The best polymer was then used to construct a platform suitable for medical use.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available