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Title: Sexual mating in Neurospora crassa
Author: Kuo, Hsiao-Che
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2008
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In N. crassa, a specialized hypha, the trichogyne, grows out chemotropically from the ascogonium (female cell) towards a sex pheromone releasing male cell which is commonly a macroconidium of opposite mating type. Following macroconidium-trichogyne fusion, the male and female nuclei were formed to be arrested in nuclear division. The female nuclei became immobilized, rounded up and clumped together whilst all of the male nuclei from the macroconidium moved unidirectionally and sequentially towards the ascogonium with an ‘inchworm-like’, repeated elongation and condensation pattern of movement. Dynein, kinesins and myosins played a role in regulating perithecial formation and the behaviour of male and female nuclei during mating. The dynein subunits DYN2, DLC, DIC and DYN27, the kinesins NKIN2 and KAR3, and the myosin MYO2 encoded by the female influenced male nuclear behaviour whilst the dynein subunit RO-3, the kinesin KIP2 and the myosins MYO1 and MYO2 encoded by male influenced female nuclear behaviour. These results showed that motor proteins derived from the male and female cooperate to regulate the movement of male nuclei and that male-female nuclear recognition occurs immediately following macroconidium-trichogyne fusion. Disruption of microtubule and actin polymerization, and inhibition of myosin activity, inhibited perithecial development and perturbed male nuclear behaviour during mating. A new type of hypha produced by conidia, the conidial sex tube (CST), was discovered. It was found to be included by sex pheromone from the opposite mating type and to be regulated by red, green and blue light.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available