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Title: The conservation and divergence of function in the SIX family of homeodomain transcription factors
Author: Kitson-Pantano, Raphaëla Joanna
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2008
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The aims of this PhD were to determine whether Drosophila is a good model for studying Six genes and to investigate the conservation and divergence of function of the different Six genes in Drosophila melanogaster. We hypothesised that the different SIX proteins were functionally distinct and that these functional differences are conserved between species. In order to test this, the ability of one Six protein to substitute for the loss of function of another Six protein was assessed through genetic rescue experiments. Similarly, the ability for a vertebrate orthologue to substitute for a mutation of a Drosophila Six gene was investigated. The ability of each of the three Six genes of Drosophila and of SIX1, SIX4 and SIX4 to complement the muscle and gonad phenotypes in a Six4 mutant was assessed. This also gave insight on the role of Six4 in mesoderm development. The first results chapter is a bioinformatics analysis of the protein sequence comparisons carried out throughout the metazoan phylum. The ability for the Drosophila melanogaster paralogues (Sine oculis and Optix) and vertebrate orthologues (Six1, Six3 and Six4) of Six4 to rescue aspects of the Six4 mutant phenotype are then discussed in the second and third results chapters.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available