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Title: Comparative structural analysis of reinnervated muscle following nerve injury and repair
Author: Kimpton, Amanda-Jane
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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The repair of peripheral nerve injuries is a major reconstructive problem, particularly when direct suturing of a transected nerve is not feasible option. This arises because mechanisms of nerve repair are not fully understood. The purpose of the first group of experiments was to compare methods of nerve repair, including use of novel, biodegradable glass tubes. The possible use of these tubes as a means of confining humoral or cellular substances at the site of repair was also assessed. In a further study, experiments were designed to establish first, the optimal timing of nerve repair (immediate or delayed); second, whether there was a difference in the level of recovery between neonates and adults after nerve repair; third, to assess the freeze-thawed muscle graft (FTMG) method as a surgical technique and to determine whether the FTMG is at least as good as a conventional nerve graft. This investigation was carried out in an animal model of obstetrical brachial plexus palsy (OBPP). Assessment of experimental outcome in both studies was by measurement of the structural and cytochemical changes which occurred in the target muscle after peripheral nerve injury and repair. Morphometric, histochemical and immunocytochemical measurements showed alterations in muscle fibre size and architecture, as well as connective tissue content and the proportions and the distributions of the different fibre types. These changes indicated that after repair with controlled-release glass tubes there was reinnervation of the target muscle, although the results were superior after repair by FTMG. As to whether the potential of nerve to regenerate after repair decreases with age, or whether immediate or delayed repair is best in the treatment of OBPP, the experiments have contributed to solving but have not resolved the dilemma associated with these issues.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available