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Title: Characterisation of immune responses of ruminants and mice to the Welgevonden stock of C. ruminantium
Author: Kibor, A. C.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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This study sought to identify antigens involved in protective immune responses to Cowdria by Western blotting using immune sera and surface labelling of elementary body (EB) proteins using biotin to identify which of these are outer membrane proteins. Antigens which could be considered as potential vaccine candidates were identified. Immunisation of goats with live Cowdria or with inactivated elementary bodies (IEBs) leads to development of antibodies to at least six antigenic components of the EB of 24kDa, 27kDa, 31kDa, 58kDa and 66kDa. In contrast immunisation of goats with detergent extracted soluble antigens stimulated production of antibodies to only four antigens of 24kDa, 27kDa, 28kDa and 31kDa. Six surface exposed antigens of the Cowdria elementary body were identified by biotin labelling, with molecular masses of 21kDa, 28kDa, 31kDa, 62kDa, 74kDa and 115kDa and are therefore considered outer membrane proteins. These proteins reacted with antibodies in sera raised by immunisation of goats with live or inactivated EBs. The 58kDa heat shock protein (GroEL) of C. ruminantium is an immunodominant antigen. The immune responses to 58kDa antigen expressed as a recombinant in E. coli were investigated by immunisation of mice and sheep. The immunised animals stimulated specific antibody which reacted with the native homologue on the EB 58kDa. Immunisation with recombinant 58kDa heat shock protein of C. ruminantium led to development a of specific antibody response of the IgG1 isotype. Challenge of immunised sheep showed a highly significant reduction (p<0.005) in infection rates of brain capillary endothelial cells in the immunised group compared to control animals. However the incubation period and clinical outcome were not significantly different in controls and immunised sheep. Mice immunised with recombinant 58kDa were partially protected against virulent homologous challenge. The lack of protection in sheep was attributed to failure of the antigen to induce a detectable lymphocyte response.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available